Journal: Bone & Joint Research

Article Title: Comparison of ligamentization potential between anterior cruciate ligament-derived cells and adipose-derived mesenchymal stem cells reseeded to acellularized tendon allograft

doi: 10.1302/2046-3758.1111.BJR-2021-0548.R2

Figure Lengend Snippet: Tendon allograft injected with anterior cruciate ligament (ACL)-derived cells, or adipose-derived mesenchymal stem cells (ADMSCs) was stained and observed by a) Masson’s trichrome staining for total collagen and b) Sirius Red staining for collagen types I and III. Magnification ×200, scale bar 200 µm. c) Immunohistochemical staining of the tendon allograft reseeded with ACL-derived cells or ADMSCs was conducted at seven days for type I collagen (upper) and type III collagen (lower). Magnification ×200, scale bar 100 µm. d) Immunofluorescence of the tendon allograft injected with ACL-derived cells or ADMSCs was conducted at day 7 for Ki-67 (red) and type I collagen (green). e) mRNA expression of ligament-specific markers (COL1, COL3, tenascin C (TNC), and tenomodulin (TNMD)), chondrogenic marker (COL2), and smooth muscle actin (SMA) was analyzed by quantitative reverse transcription polymerase chain reaction (n = 3). DAPI, 4′,6-diamidino-2-phenylindole; mRNA, messenger RNA.

Article Snippet: The antibodies for IHC were as follows: COL1A1 (Santa Cruz Biotechnology, USA, 8784; dilution, 1:200) and COL3 (ABclonal Technology, USA, a3795; dilution, 1:200).

Techniques: Injection, Derivative Assay, Staining, Immunohistochemical staining, Immunofluorescence, Expressing, Marker, Reverse Transcription Polymerase Chain Reaction

Journal: Materials Today Bio

Article Title: Engineered decellularized tendon hydrogel with sustained zinc ion release orchestrates anti-inflammatory microenvironment and functional regeneration in Achilles tendinopathy

doi: 10.1016/j.mtbio.2025.102104

Figure Lengend Snippet: Schematic illustration of Z-D hydrogel's dual functions in modulating the inflammatory microenvironment and facilitating tendon regeneration. The released ZnO NPs from Z-D hydrogel attenuate inflammation through inhibition of IL-6 and TNF-α expression, while promoting tendon repair by upregulating SCX, TNMD, and COL I expression alongside downregulation of COL III.(SCX:scleraxis; TNMD: tenomodulin; COL I:type I collagen; COL III: type III collagen; IL-6: interleukin-6; TNF-α: tumor necrosis factor-α).

Article Snippet: All stained sections were mounted with neutral resin and examined under a light microscope equipped with digital imaging capabilities, with particular attention to cellular infiltration, collagen alignment, and vascularization patterns.The immunohistochemical workflow involved sequential steps of slide deparaffinization, antigen retrieval via citrate buffer (Solarbio, China) microwave treatment, endogenous peroxidase blockade with 3 % H 2 O 2 (10 min), 37 °C trypsin digestion (2 h), and 10 % goat serum blocking, Followed this step, the sections were subjected to overnight incubation at 4 °C with primary antibodies targeting SCX (PA5-115874, Invitrogen), TNMD (ab203676, Abcam), COL I (A16891, Proteintech), COL III (A3795, Proteintech), IL-6 (21865-1-AP, Proteintech), TNF-α (21865-1-AP, Proteintech), CD86(13395-1-AP,Proteintech)and CD206(18704-1-AP,Proteintech).

Techniques: Inhibition, Expressing

Journal: Materials Today Bio

Article Title: Engineered decellularized tendon hydrogel with sustained zinc ion release orchestrates anti-inflammatory microenvironment and functional regeneration in Achilles tendinopathy

doi: 10.1016/j.mtbio.2025.102104

Figure Lengend Snippet: Histological analysis. (A) HE staining; (B) Masson staining; (C) Immunohistochemistry of SCX in each group at early repair stage (1 week) and late stage (4 weeks); (D) Immunohistochemistry of TNMD expression changes. (E) Immunohistochemistry of COL I during injury repair; (F) Immunohistochemistry of COL III during injury repair.

Article Snippet: All stained sections were mounted with neutral resin and examined under a light microscope equipped with digital imaging capabilities, with particular attention to cellular infiltration, collagen alignment, and vascularization patterns.The immunohistochemical workflow involved sequential steps of slide deparaffinization, antigen retrieval via citrate buffer (Solarbio, China) microwave treatment, endogenous peroxidase blockade with 3 % H 2 O 2 (10 min), 37 °C trypsin digestion (2 h), and 10 % goat serum blocking, Followed this step, the sections were subjected to overnight incubation at 4 °C with primary antibodies targeting SCX (PA5-115874, Invitrogen), TNMD (ab203676, Abcam), COL I (A16891, Proteintech), COL III (A3795, Proteintech), IL-6 (21865-1-AP, Proteintech), TNF-α (21865-1-AP, Proteintech), CD86(13395-1-AP,Proteintech)and CD206(18704-1-AP,Proteintech).

Techniques: Staining, Immunohistochemistry, Expressing